RT journal article
T1 Microalgal and Cyanobacterial Biomasses Modified the Activity of Extracellular Products from Bacillus pumilus: An In Vitro and In Vivo Assessment
A1 García-Márquez, Jorge
A1 Díaz, A.G.
A1 Molina Roque, Luis
A1 Domínguez-Maqueda, M.
A1 Heras Rodríguez, Veronica de las
A1 Simo Mirabet, Paula
A1 Vizcaíno, A.J.
A1 Martos Sitcha, Juan Antonio
A1 Alarcón-López, F.J.
A1 Moriñigo, M.A.
A1 Balebona, M.C.
A2 Biología
K1 3R principle
K1 Aquaculture
K1 Extracellular products
K1 Microalgae
K1 Sparus aurata
AB This study investigates the postbiotic potential of extracellular products (ECPs) from Bacillus pumilus strains cultivatedon microalgae-supplemented media. We assessed enzymatic and antimicrobial activities to select ECPs that enhance thedigestive processes in gilthead seabream. Additionally, we explored the in vitro enzymatic capacity of the chosen postbioticsto hydrolyze macromolecules in microalgae. Finally, a feeding trial was conducted to determine the in vivo effects of theECPs on Sparus aurata. In vitro enzymatic assays demonstrated diverse hydrolytic capacities among ECPs. All conditionsexhibited antimicrobial activity against Photobacterium damselae subsp. piscicida, with variation in inhibitory effects againstVibrio harveyi and Tenacibaculum maritimum. Furthermore, in vitro assays revealed differences in protein hydrolysis andsoluble protein concentration, influencing amino acid and reducing sugar release from microalgal biomass. These analysesfacilitated a selection to test ECPs in vivo. Lastly, the in vivo experiment revealed no differences in the growth performance,nutrient utilization, and general metabolism of S. aurata fed the experimental diets. Dietary inclusion of postbiotics increasedthe activity of key digestive enzymes in fish compared to the control group, and particularly, values increased significantlywhen the fish were fed with the ECP-nanoparticulate-supplemented diet. In conclusion, the inclusion of microalgae in theculture media significantly influences the activity of extracellular products from B. pumilus strains, as evidenced in bothin vitro and in vivo assays.
PB Springer
SN 1867-1314
YR 2024
FD 2024
LK http://hdl.handle.net/10498/35549
UL http://hdl.handle.net/10498/35549
LA eng
NO Funding for open access publishing: Universidad Málaga/CBUA. Funding for open access charge: Universidad de Málaga / CBUA. This work was funded by the Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI) from the Junta de Andalucía (Spain) (#P20_00085). This work has also been funded by the Spanish Ministry of Sciences, Innovation, and Universities (#PID2020-113637RB-C22). This research was co-founded by the projects HYDROALGAE4BREAM (PID2021-12228OBC21) from the Ministry of Sciences, Innovation, and Universities (Spain) and by the European Union under the 2014–2020 ERDF Operational Programme and AquaTech4Feed project (grant # PCI2020-112204 by AEI within the ERA-NET BioBlue COFUND). The authors thank grants UNAM15-CE-3510, EQC2018-004984-P, and EQC2019-006380-P to Service of Experimental Diets. This work was co-funded by the European Union under the 2014–2020 ERDF Operational Programme and by the Regional Government of Andalusia (Project reference: FEDER-UCA18-107182). P. Simó-Mirabet is supported by a Postdoctoral Research Fellowship (Juan de la Cierva-Formación, Reference FJCI-2021–047759-I) from the Spanish Ministry of Science and Innovation.
DS Repositorio Institucional de la Universidad de Cádiz
RD 22-sep-2026