RT journal article
T1 Unraveling the differential mechanisms of revascularization promoted by MSCs & ECFCs from adipose tissue or umbilical cord in a murine model of critical limb-threatening ischemia
A1 Rojas Torres, Marta
A1 Beltrán Camacho, Lucía
A1 Martínez Val, Ana
A1 Sánchez Gomar, Ismael
A1 Eslava Alcón, Sara
A1 Rosal Vela, Antonio
A1 Jiménez Palomares, Margarita
A1 Doiz Artázcoz, Esther
A1 Martínez-Torija, Mario
A1 Moreno-Luna, Rafael
A1 Olsen, Jesper V.
A1 Durán Ruiz, María del Carmen
A2 BiomedicinaBiotecnología y Salud Pública
A2 Cirugía
AB AbstractBackground Critical limb-threatening ischemia (CLTI) constitutes the most severe manifestation of peripheral arterydisease, usually induced by atherosclerosis. CLTI patients suffer from high risk of amputation of the lower extremitiesand elevated mortality rates, while they have low options for surgical revascularization due to associated comorbidities.Alternatively, cell-based therapeutic strategies represent an effective and safe approach to promote revascularization.However, the variability seen in several factors such as cell combinations or doses applied, have limited theirsuccess in clinical trials, being necessary to reach a consensus regarding the optimal “cellular-cocktail” prior furtherapplication into the clinic. To achieve so, it is essential to understand the mechanisms by which these cells exert theirregenerative properties.Herein, we have evaluated, for the first time, the regenerative and vasculogenic potential of a combinationof endothelial colony forming cells (ECFCs) and mesenchymal stem cells (MSCs) isolated from adipose-tissue (AT),compared with ECFCs from umbilical cord blood (CB-ECFCs) and AT-MSCs, in a murine model of CLTI.Methods Balb-c nude mice (n:32) were distributed in four different groups (n:8/group): control shams, and ischemicmice (after femoral ligation) that received 50 μl of physiological serum alone or a cellular combination of ATMSCswith either CB-ECFCs or AT-ECFCs. Follow-up of blood flow reperfusion and ischemic symptoms was carriedout for 21 days, when mice were sacrificed to evaluate vascular density formation. Moreover, the long-term molecularchanges in response to CLTI and both cell combinations were analyzed in a proteomic quantitative approach.Results AT-MSCs with either AT- or CB-ECFCs, promoted a significant recovery of blood flow in CLTI mice 21 dayspost-ischemia. Besides, they modulated the inflammatory and necrotic related processes, although the CB group
SN 1423-0127
YR 2024
FD 2024-07-15
LK http://hdl.handle.net/10498/35703
UL http://hdl.handle.net/10498/35703
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026