RT journal article
T1 Enhancing farmed fish welfare: Evaluating the effectiveness of plant-based stress mitigating agents as sedatives in sea bass (Dicentrarchus labrax) following intraperitoneal vaccination
A1 Azizi, S.
A1 Balasch, J.C.
A1 Cartan Moya, Sara
A1 Jerez Cepa, Ismael
A1 Mancera Romero, Juan Miguel
A1 Tort, L.
A1 Khansari, A. R.
A2 Biología
K1 Vaccination
K1 Immune response
K1 Stress response
K1 Essential oils
AB The effectiveness of vaccines may be compromised by the stress response induced by intraperitoneal/intramuscular (IP/IM) vaccination due to an intimate interaction between the neuroendocrine and immune systems.Essential oils (EOs), known for their antibacterial, antioxidant, and sedative properties, are potential candidatesto mitigate this stress response. This study investigates the short-term sedative effect of two essential oil-basedproducts, FishEase-C (FEC) and FishEase-L (FEL) in sea bass prior to intraperitoneal vaccination (IP). Physiological stress indicators (plasma cortisol, glucose, lactate) increased 1-h post-vaccination (hpv) as expected.Cortisol remained elevated up to 24 hpv in the N.P. vaccine and FEC + vaccine groups but decreased with FELtreatment (FEL + vaccine group). However, FEC at the tested concentration appeared to induce stress. Thetranscription of stress (gr, hsp70, cox2), immune (il1β, il6, tnfα), and antioxidant (gpx, sod, catalase) genesconfirmed the vaccination-induced stress response, with mc2r transcription indicating increased cortisol production in vaccinated groups (N.P. vaccine and FEC + vaccine). FEL reduced stress at both physiological (e.g.,cortisol) and transcriptional levels (e.g., hsp70, cox2 and il6) at either 1 or 24 hpv. It is worth noting that, from aninflammatory perspective, there was a big difference between tissues in terms of magnitude and pattern(treatment and time effects). The brain was more resistant to inflammation, while the head kidney and spleenshowed heightened il1β expression (860-2100-fold). These findings support the use of FEL as a sedative beforeIP/IM vaccination in sea bass.
PB Elsevier
SN 1095-9947
YR 2024
FD 2024-11-28
LK http://hdl.handle.net/10498/35731
UL http://hdl.handle.net/10498/35731
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026