RT journal article
T1 Dark Fermentation of Fruit Juice Effluents: Effects of  Substrate Concentration
A1 Díaz Domínguez, Encarnación
A1 Ibáñez López, María Eugenia
A1 García Morales, José Luis
A1 López-Fernández, Ester
A1 Fernandez-Morales, Francisco Jesus
A2 Tecnologías del Medio Ambiente
K1 acidogenic fermentation
K1 hydrogen production
K1 volatile fatty acids
K1 inhibition
AB This study explores the impact of initial substrate concentrations on biomass growth, hydrogen production, and acid generation during acidogenic fermentation of a synthetic fruit juice wastewater. Four substrate concentrations, within the range 0.30–2.12 C mol·L−1, were tested using a mixed acidogenic bacterial culture. Batch reactors were employed to conduct the study, and the results were analyzed to determine inhibition effects. The highest hydrogen production (6.1 L H2·substrate C mol−1) and hydrogen percentage in the gas phase (57%) were achieved at a substrate concentration of 0.30 C mol·L−1. Higher substrate concentrations reduced the hydrogen production due to substrate and product inhibition events. The maximum H2 potential production was 4.15 Nm3/m3 reactor at a substrate concentration of 0.91 C mol·L−1. Biomass growth and VFA production followed exponential trends at low substrate concentrations, 0.30 and 0.091 C mol·L−1, while high concentrations resulted in linear trends due to inhibition effects caused by the substrate. The main acids produced were lactic at low concentrations, and acetic when dealing with high concentrations. The highest final acid concentrations were obtained with the highest initial substrate concentration, but their yields were significantly lower due to the substrate and product inhibitions experienced by the biomass.
PB MDPI
SN 2076-3417
YR 2024
FD 2024-11-15
LK http://hdl.handle.net/10498/35761
UL http://hdl.handle.net/10498/35761
LA eng
NO This research was funded by the European Union within the framework of the ERDF Operational Program 2014–2020 and by the Ministry of Economic Transformation, Industry, Knowledge and Universities of the Junta de Andalucía. Project reference: FEDER-UCA18-107460. Co-financing of the Government of Spain and from Junta de Comunidades de Castilla-La Mancha Project BPLY/23/180225/000143. The authors thank the predoctoral contract of the Junta de Andalucía PREDOC-01870 (Encarnación Díaz-Domínguez).
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026