RT journal article
T1 Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
A1 Robles-Arozarena, R.
A1 Martos Sitcha, Juan Antonio
A1 Bermúdez, Laura
A1 Nuez-Ortín, Waldo
A1 Fuentes, Juan
A2 Biología
K1 Sea bream
K1 Somatic indices
K1 Fishmeal
K1 Electrophysiology
K1 Intestinal transport
K1 Lysophospholipids
K1 Ussing chamber
AB In response to sustainability concerns related to the origin of raw materials, aquaculture has emphasized the useof additives and enhancers to improve the intestinal absorption of proteins and amino acids. However, the toolsavailable to have a good proxy for potential intestinal absorption and to expose positive or negative effects arescarce. The present study had a two-fold objective: we first aimed to characterize electrogenic essential aminoacid transport in the intestine of the gilthead seabream ex-vivo. We also aimed to understand if dietary lysophospholipids (LPLs) had positive or detrimental effects when incorporated into commercial diets in giltheadseabream (Sparus aurata) juveniles. The ex-vivo characterization in Ussing chambers showed a region-dependentelectrogenic amino acid transport in the sea bream intestine, being maxima in the mid intestine and with a strongluminal sodium- and pH-dependence. In vivo, the animals were divided into two groups: one was fed a controldiet (10 % fishmeal inclusion), whereas the other group was fed with the control diet supplemented with 0.1 %LPL in coated form. The LPL-inclusion was without effects on fish growth in a short-term feeding trial, althoughthere was a significant improvement of hepatosomatic and viscerosomatic indices. Interestingly, the intestine offish fed the aquafeed with a 0.1 % LPL supplementation increased paracellular permeability as measured in theanterior intestine and enhanced electrogenic amino acid transport in the mid intestine. Our results stronglysuggest that the full potential of LPL-inclusion benefits could be explored in sustainable diets, nearly devoid offishmeal and/or in fish subjected to environmental stress
PB Elsevier
SN 2352-5134
YR 2024
FD 2024-12-02
LK http://hdl.handle.net/10498/35800
UL http://hdl.handle.net/10498/35800
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 22-sep-2026