RT journal article
T1 High plant protein diet impairs growth performance and intestinal integrity in greater amberjack (Seriola dumerili): Molecular and physiological insights
A1 Fuentes, Juan
A1 Fonseca, Filomena
A1 Filipa Gregório, Silvia
A1 Kussaba, Larissa
A1 Perera Bravet, Erick
A1 Alarcón López, Francisco Javier
A1 Martos Sitcha, Juan Antonio
A2 Biología
K1 Greater amberjack
K1 Fishmeal replacement
K1 Intestine
K1 Integrity
K1 Amino acid
AB The Mediterranean aquaculture industry that produces mainly seabream and seabass is exploring alternativeplant-based protein sources for fish feeds together with the diversification of fish species. Effective plant-basedformulations require diverse sources and additives to maintain fish welfare and growth, which is especiallyimportant for carnivorous species. In this scenario, the objective of the present study was to evaluate the impactof a high percentage of fishmeal replacement with protein of plant origin in Seriola dumerili, a fast-growingspecies with a high protein requirement. Two diets were developed: MAP (marine animal protein) with a protein content of 92 % of animal origin and PPB (plant protein blend) with a 50 % replacement of protein of animalorigin with plant protein. We combined electrophysiology measurements with expression analysis of claudinsand members of the SLC superfamily of solute carriers to unravel and characterise putative markers of intestinalintegrity and absorption. The replacement resulted in shorter and lighter fish with a reduction in growth rate(SGR) from 2.6 to 2. We simultaneously observed lower transepithelial tissue resistance (TER), lower permeability, and decreased cld12 expression in the anterior intestine. In addition, we demonstrated a strong regiondependent electrogenic transport of essential amino acids, with the mid-intestine having the highest transportcapacity. The comparative study performed in the mid-intestine with fish fed the MAP or the PPB diets exposed anegative effect of dietary replacement with plant protein. No significant dietary impact on di- and tri-peptidetransporters (SLC15) was found at the molecular level. However, a clear region-dependent expression patternof slc15a1, slc15a2, and slc15a4 was observed, which warrants further investigation. The expression pattern ofslc7a5 showed the effect of the diet, but in contrast, the diet and intestinal region affected the expression of itsfunctionally associated slc3a1 and slc3a2 for amino acid antiport, with the strongest effects in the posteriorintestine. The integrity and absorption impairments detected in response to protein source replacement willlikely underlie the significant growth differences observed between the two dietary regimes.
PB Elsevier
SN 0044-8486
YR 2024
FD 2024-11-20
LK http://hdl.handle.net/10498/35817
UL http://hdl.handle.net/10498/35817
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026