RT journal article
T1 Enhancing Cellulose and Lignin Fractionation from Acacia Wood: Optimized Parameters Using a Deep Eutectic Solvent System and Solvent Recovery
A1 Aliaño González, María José
A1 Rodrigues, Mariana
A1 Fernandes, Catarina
A1 Mendes, Cátia V. T.
A1 Carvalho, Maria Graça V. S.
A1 Alves, Luís
A1 Medronho, Bruno
A1 Rasteiro, Maria da Graça
A2 Química Analítica
K1 biomass fractionation
K1 deep eutectic solvents
K1 Box–Behnken design
K1 acacia wood
K1 lignocellulose
AB Cellulose and lignin, sourced from biomass, hold potential for innovative bioprocesses and biomaterials. However, traditional fractionation and purification methods often rely on harmful chemicals and high temperatures, making these processes both hazardous and costly. This study introduces a sustainable approach for fractionating acacia wood, focusing on both cellulose and lignin extraction using a deep eutectic solvent (DES) composed of choline chloride (ChCl) and levulinic acid (LA). A design of experiment was employed for the optimization of the most relevant fractionation parameters: time and temperature. In the case of the lignin, both parameters were found to be significant variables in the fractionation process (p-values of 0.0128 and 0.0319 for time and temperature, respectively), with a positive influence. Likewise, in the cellulose case, time and temperature also demonstrated a positive effect, with p-values of 0.0103 and 0.028, respectively. An optimization study was finally conducted to determine the maximum fractionation yield of lignin and cellulose. The optimized conditions were found to be 15% (w/v) of the wood sample in 1:3 ChCl:LA under a treatment temperature of 160 °C for 8 h. The developed method was validated through repeatability and intermediate precision studies, which yielded a coefficient of variation lower than 5%. The recovery and reuse of DES were successfully evaluated, revealing remarkable fractionation yields even after five cycles. This work demonstrates the feasibility of selectively extracting lignin and cellulose from woody biomass using a sustainable solvent, thus paving the way for valorization of invasive species biomass.
PB MDPI
SN 1420-3049
YR 2024
FD 2024-07-25
LK http://hdl.handle.net/10498/35913
UL http://hdl.handle.net/10498/35913
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026