RT journal article
T1 New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe
A1 García Guzmán, Juan José
A1 Jiménez Heras, José Manuel
A1 López-Iglesias, David
A1 González-Álvarez, Rafael J.
A1 Cubillana Aguilera, Laura
A1 González Macías, María del Carmen
A1 Fernández Alba, Juan Jesús
A1 Palacios Santander, José María
A2 Materno-Infantil y Radiología
A2 Química Analítica
K1 Lactate amperometric biosensor
K1 Diffusion limiting membrane
K1 Spin coating procedure
K1 Continuous monitoring
K1 Healthcare assessment
AB A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of thebiosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfacesin comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensorfor lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes rangingfrom 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum sampleswere estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensordeveloped is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on commonpolyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leadingto excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmedby using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could beachieved in continuous flow regime using both (bio)sensors.
PB Springer
SN 1436-5073
YR 2024
FD 2024-08-09
LK http://hdl.handle.net/10498/35927
UL http://hdl.handle.net/10498/35927
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026