RT journal article
T1 RNA Interference Applied to Crustacean Aquaculture
A1 Fajardo Quiñones, Carlos
A1 De Donato, Marcos
A1 Macedo, Marta
A1 Charoonnart, Patai
A1 Saksmerprome, Vanvimon
A1 Yang, Luyao
A1 Purton, Saul
A1 Costas, Benjamín
A2 Biología
K1 anti-viral
K1 dsRNA
K1 gene-silencing
K1 immunology
K1 innate immunity
K1 miRNA
K1 nanoencapsulation
K1 RNAi
K1 shrimp
K1 siRNA
AB RNA interference (RNAi) is a powerful tool that can be used to specifically knock-down gene expression using double-stranded RNA (dsRNA) effector molecules. This approach can be used in aquaculture as an investigation instrument and to improve the immune responses against viral pathogens, among other applications. Although this method was first described in shrimp in the mid-2000s, at present, no practical approach has been developed for the use of dsRNA in shrimp farms, as the limiting factor for farm-scale usage in the aquaculture sector is the lack of cost-effective and simple dsRNA synthesis and administration procedures. Despite these limitations, different RNAi-based approaches have been successfully tested at the laboratory level, with a particular focus on shrimp. The use of RNAi technology is particularly attractive for the shrimp industry because crustaceans do not have an adaptive immune system, making traditional vaccination methods unfeasible. This review summarizes recent studies and the state-of-the-art on the mechanism of action, design, use, and administration methods of dsRNA, as applied to shrimp. In addition, potential constraints that may hinder the deployment of RNAi-based methods in the crustacean aquaculture sector are considered.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2218-273X
YR 2024
FD 2024
LK http://hdl.handle.net/10498/36003
UL http://hdl.handle.net/10498/36003
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026