RT journal article T1 Lutein enrichment of the rotifer Brachionus sp. using freeze-dried Muriellopsis sp. cells A1 van Bergeijk, Stefanie Anne A1 Paullada Salmerón, José Antonio A1 López-Pérez, Ana Isabel A1 Moreno, José A1 Cañavate, José Pedro A1 García-González, Mercedes A2 Biología K1 Muriellopsis sp. K1 microalga K1 lutein K1 rotifer enrichment K1 Brachionus sp. AB Rotifers, used in aquaculture as feed for early larval stages, are poor in carotenoids compared with cope- pods, the natural most common prey item of marine fish larvae. A minimal sufficient level of carotenoids in fish larvae may be essential for a correct develop- ment. The freshwater microalga Muriellopsis sp. has been identified as a potential source of the caroten- oid lutein with possible application in aquaculture. Lutein is a potent antioxidant, with potentially ben- eficial effects as a carotenoid for fish larvae. This study describes the lutein enrichment of the rotifer Brachionus sp. when incubated with homogeneously dispersed suspensions of freeze-dried, lutein-rich Muriellopsis sp. in seawater. A short enrichment time (2 or 2.5 h) resulted in a higher lutein content of the rotifers than a long enrichment time (13.5 or 24 h). Although the concentration of lutein in enriched rotifers, 0.06–0.16 mg (g dry weight) 1 was low compared with its content in Muriellopsis sp. [about 3 mg (g dry weight) 1], it was in the range reported for natural copepod populations. Therefore, we conclude that freeze-dried biomass of Muriellopsis sp. can be used to enrich rotifers with lutein, providing an adequate level of this caroten- oid for marine fish larvae. PB Wiley SN 13652109 YR 2013 FD 2013-10-10 LK http://hdl.handle.net/10498/36279 UL http://hdl.handle.net/10498/36279 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 08-nov-2026