RT journal article T1 Evaluation of the Sedative Effect of Limonene to Reduce Stress During Transportation of Gilthead Seabream (Sparus aurata) A1 Simo Mirabet, Paula A1 Caderno Peña, Anyell A1 Flores-Llano, M.J. A1 da Silva, E.G. A1 Schoenau, W. A1 Baldisserotto, Bernardo A1 Martínez Rodríguez, Gonzalo A1 Mancera Romero, Juan Miguel A1 Martos Sitcha, Juan Antonio A2 Biología K1 aquaculture procedures K1 essential oils K1 fish transportation K1 HPI axis K1 welfare AB Common aquaculture practices such as fish transport can induce stress. With interest in animal welfare increasing among consumers as well as for the industry, the main objective of this study was to evaluate the sedative action of limonene to reduce stress during fish transportation in an important commercial species such as Sparus aurata. After the optimal dose of limonene was determined through measurements of the reduction in respiratory rate, fish transported for 6 h with the sedative showed higher water oxygen levels together with a more homeostatic state, judged by lower changes in osmolality or stress parameters (plasma cortisol and glucose and gene expression of stress biomarkers in the head kidney), than fish transported without limonene. Limonene-treated fish also presented a reduced metabolism, which may suggest a more relaxed state. However, the sedative benefits of limonene were partly masked by the negative effects of its solvent (ethanol); therefore, finding an alternative could enhance the potential of limonene as a sedative for fish transport. The findings of this research could help reduce fish stress derived from aquaculture practices, benefiting both fish and the industry. PB Multidisciplinary Digital Publishing Institute (MDPI) SN 2079-7737 YR 2025 FD 2025 LK http://hdl.handle.net/10498/36380 UL http://hdl.handle.net/10498/36380 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 21-sep-2026