RT journal article T1 Hake Fish Preservation Using Plant-Based Impregnated Polylactic Acid Food Films as Active Packaging A1 Sánchez García, Josefa A1 Machado, Noelia Daiana A1 Tirado Fernández, María A1 Cejudo Bastante, Cristina A1 Roldán Gómez, Ana María A1 Mantell Serrano, Casimiro A1 Casas Cardoso, Lourdes A2 Ingeniería Química y Tecnología de Alimentos K1 hake fish K1 polylactic acid K1 olive leaf extract K1 supercritical fluids AB Global fish consumption has steadily increased; however, fishery productsare difficult to preserve. Active packaging has emerged as an alternative to improve itsconservation. In this work, fresh hake fillets were packaged in commercial polylacticacid films impregnated with olive leaf extract using supercritical CO2. The impregnationwas performed at 35 ◦C and 400 bar for 1 h. The ABTS assay was used to determine theantioxidant activity, and migration tests were performed using food simulants A and D2 for10 days at 5 ◦C. The fresh fillets were packaged in impregnated and control films and storedfor 12 days at 4 ◦C. The microbiological, physical (drip loss, aw, pH, and color) and chemicalparameters (total volatile base and trimethylamine) were analyzed. The impregnatedfilms presented a 706 µg extract mg−1 polymer, showing a 2-fold extract release usingfood simulant D2 than simulant A. After hake storage using impregnated films, reducedmicrobial count, and drip loss, maintaining the pH stability was obtained. The color turnedyellowish and no detectable olfactory presence of the extract was noted. The chemicalparameters were similar in both types of films. The proposed biodegradable packagingwith olive by-products preserves moisture and controls microbial growth, representing aneco-friendly alternative. PB MDPI SN 2076-3417 YR 2025 FD 2025 LK http://hdl.handle.net/10498/36425 UL http://hdl.handle.net/10498/36425 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 10-nov-2026