RT journal article
T1 Insights into Solea senegalensis Reproduction Through Gonadal Tissue Methylation Analysis and Transcriptomic Integration
A1 Ramírez Torres, Daniel
A1 Anaya Romero, Marco
A1 Rodríguez Jiménez, María Esther
A1 Arias Pérez, Alberto
A1 Mukiibi, Robert
A1 D’Cotta, Helena
A1 Robledo, Diego
A1 Rebordinos González, Laureana
A2 BiomedicinaBiotecnología y Salud Pública
K1 Solea senegalensis
K1 flatfish
K1 reproduction
K1 methylation
K1 transcriptome
AB Fish exhibit diverse mechanisms of sex differentiation and determination, shapedby both external and internal influences, often regulated by distinct DNA methylationpatterns responding to environmental changes. In S. senegalensis aquaculture, reproductiveissues in captivity pose significant challenges, particularly the lack of fertilization capabilities in captive-bred males, hindering genetic improvement measures. This study analyzedthe methylation patterns and transcriptomic profiles in gonadal tissue DNA from groupsdiffering in rearing conditions and sexual maturity stages. RRBS (Reduced Representation Bisulfite Sequencing) was employed to detect notable methylation variations acrossgroups, while RNA was extracted and sequenced for differential expression analysis. Ourfindings suggest that DNA methylation significantly regulates gene expression, acting as amechanism that can both repress and enhance gene expression depending on the genomiccontext. The complexity of this epigenetic mechanism is evident from the varying levels ofmethylation and correlation rates observed in different CpGs neighboring specific geneslinked to reproduction. Differential methylation comparisons revealed the highest numberof differently methylated CpGs between maturation stages, followed by rearing conditions,and lastly between sexes. These findings underscore the crucial role of methylation inregulating gene expression and its potential role in sex differentiation, highlighting thecomplex interplay between epigenetic modifications and gene expression.
PB MDPI
SN 2218-273X
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36449
UL http://hdl.handle.net/10498/36449
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026