RT journal article
T1 Validity and reliability of V˙O2max determination immediately after a maximal fat oxidation assessment in pre-diabetes/type 2 Diabetes Mellitus population
A1 Marín Galindo, Alberto
A1 Opazo Díaz, Edgardo
A1 Ramírez Jiménez, Miguel
A1 Montes de Oca García, Adrian
A1 Corral Pérez, Juan
A1 Pérez Pérez, Alejandro
A1 Casals Vázquez, Cristina
A1 Ponce González, Jesús Gustavo
A2 Didáctica de la Educación FísicaPlástica y Musical
K1 Validation
K1 Cardiorespiratory fitness
K1 FatMax
K1 Metabolic flexibility
K1 Exercise tests
K1 Exercise physiology
AB Background: Measuring maximal fat oxidation (MFO) and maximal oxygen consumption (V_ O2max) typically requires two separate days, which can be time-consuming. This study aimed to assess the validity and reliability of asingle-day test for measuring MFO and V_ O2max in individuals with pre-diabetes/type 2 diabetes (T2DM).Methods: Twenty adults with pre-diabetes/T2DM (10 women; [53.1   8.8] years) participated in this crosssectional study. Participants underwent a single-day MFO- V_ O2max protocol, followed by the traditional V_ O2maxtest on a cycle ergometer 4–7 days later, with both assessments performed using indirect calorimetry. The singleday test commenced with the MFO test, involving 15 W per 3 min increments until the respiratory exchange ratereached 1.0. After a 5-minute (min) rest, the V_ O2max test was conducted, starting at the last load and increasing by15 W⋅min-1 until voluntary exhaustion. The traditional V_ O2max test involved a 15 W⋅min-1n incremental protocoluntil voluntary exhaustion, followed by a verification test at 110% of the previous maximum load.Results: No significant differences were observed between MFO-V_ O2max and traditional V_ O2max protocol in V_ O2maxvalues ([2 099.4   534.1] ml⋅min-1 vs. [2 119.1   548.3] ml⋅min-1, p ¼ 0.715) or heart rate ([163.2   18.2] beat/min vs. [164.5   16.1] beat/min, p ¼ 0.485). The MFO-V_ O2max protocol test demonstrated excellent reliability(intraclass correlation coefficient ¼ 0.957), limits of agreement ranging from 300.06 ml⋅min-1 to 306.77 ml⋅min-1,and the standard error of measurement was 181.67 ml⋅min-1.Conclusions: Therefore, the single-day test is a valid and reliable method for assessing both MFO and V_ O2max inmen and women with pre-diabetes/T2DM. With a mean total duration of (34   8) min, this test enhances clinicalapplicability by significantly reducing the time and cost associated with traditional assessments.
PB KeAi
SN 2666-3376
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36492
UL http://hdl.handle.net/10498/36492
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026