RT journal article
T1 A microtiter plate assay as a reliable method to assure the identification and classification of the veil-forming yeasts during Sherry wines ageing
A1 Ruiz Muñoz, Marina
A1 Bernal Grande, María del Carmen
A1 Cordero Bueso, Gustavo Adolfo
A1 González, Mónica
A1 Hughes Herrera, David
A1 Cantoral Fernández, Jesús Manuel
A2 BiomedicinaBiotecnología y Salud Pública
K1 flor yeast
K1 microplate
K1 fermentation
K1 assimilation
K1 Saccharomyces cerevisiae
K1 non-Saccharomyces
K1 velum of flor
AB Yeasts involved in veil formation during biological ageing of Sherry wines are mainlySaccharomyces cerevisiae, and they have traditionally been divided into four races or varieties:beticus, cheresiensis, montuliensis and rouxii. Recent progress in molecular biology has led tothe development of several techniques for yeast identification, based on similarity or dissimilarityof DNA, RNA or proteins. In view of the latest yeast taxonomy, there are no more races. However,molecular techniques are not enough to understand the real veil-forming yeast diversity and dynamicsin Sherry wines. We propose a reliable method, using a microtiter reader, to evaluate the fermentationand assimilation of carbon and nitrogen sources, the osmotolerance and the antibiotic resistance,using 18 S. cerevisiae and 5 non-Saccharomyces yeast strains, to allow correct identification andclassification of the yeast strains present in the velum of flor complex.
PB MDPI
SN 2311-5637
YR 2017
FD 2017
LK http://hdl.handle.net/10498/36511
UL http://hdl.handle.net/10498/36511
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026