RT journal article
T1 Photoperiod and Light Spectrum Modulate Daily Rhythms and Expression of Genes Involved in Cell Proliferation, DNA Repair, Apoptosis and Oxidative Stress in a Seabream Embryonic Stem Cell Line
A1 Vergés Castillo, Alba
A1 Herrera Pérez, Patricia
A1 Pendón Meléndez, Carlos
A1 Martín Robles, Agueda Jimena
A1 Muñoz Cueto, José Antonio
A2 Biología
A2 BiomedicinaBiotecnología y Salud Pública
K1 Embryonic stem cell lines
K1 Circadian rhythms
K1 Cell cycle
K1 Photoperiod
K1 Light spectrum
K1 Teleost fish
AB The use of cell lines as alternative models for environmental physiology studies opens a new window of possibilities and is becoming an increasingly used tool in marine research to fulfil the 3R’s rule. In this study, an embryonic monoclonal stem cell line obtained from a marine teleost (gilthead seabream, Sparus aurata) was employed to assess the effects of photoperiod (light/dark cycles vs constant dark) and light spectrum (white, blue, green, blue/green and red lights) on gene expression and rhythms of cellular markers of proliferation, DNA repair, apoptosis and cellular/oxidative stress by RT-qPCR and cosinor analyses. The results obtained revealed the optimal performance of cells under blue light (LDB), with all the genes analysed showing their highest RNA expression levels and most robust daily variations/rhythms in this condition. Under LDB, the mRNA levels of cell proliferation (pcna), DNA repair (cry5), anti-apoptotic (bcl2) and oxidative stress (prdx2) markers peaked at the day-night transition, whereas pro-apoptotic (bax) and cell stress (hsp70) markers showed their highest expression at the night-day transition, evidencing the strong synchronisation of the transcription of key genes involved in the cell cycle in this photoregime. The persistence of significant pcna, cry5, hsp70 and prdx2 rhythms after 3 days in constant darkness reveals the endogenous and circadian nature of these rhythms. Our results highlight the importance of implementing photoperiods with light–dark cycles of blue wavelengths when performing fish cell culture research. These results reinforce and extend our previous studies, confirming the importance of lighting conditions that mimic the natural environment for the proper development of fish embryos and larvae in aquaculture.
PB Springer
SN 1436-2236
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36514
UL http://hdl.handle.net/10498/36514
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026