RT journal article
T1 Carbohydrates digestion and metabolism in the spiny lobster (Panulirus argus): Biochemical indication for limited carbohydrate utilization
A1 Rodríguez Viera, Leandro
A1 Perera Bravet, Erick
A1 Montero Alejo, Vivian
A1 Perdomo Morales, Rolando
A1 García Galano, Tsai
A1 Martínez Rodríguez, Gonzalo
A1 Mancera Romero, Juan Miguel
A2 Biología
K1 α-amylase
K1 Carbohydrate
K1 Energy metabolism
K1 Crustaceans
K1 Glycemia
K1 Gene expression
K1 Panulirus argus
K1 Lobster
K1 Carbohydrate digestion
AB As other spiny lobsters, Panulirus argus is supposed to use preferentially proteins and lipids in energy metabolism, while carbohydrates are well digested but poorly utilized. The aim of this study was to evaluate the effect of dietary carbohydrate level on digestion and metabolism in the spiny lobster P. argus. We used complementary methodologies such as post-feeding flux of nutrients and metabolites, as well as measurements of α-amylase expression and activity in the digestive tract. Lobsters readily digested and absorbed carbohydrates with a time-course that is dependent on their content in diet. Lobster showed higher levels of free glucose and stored glycogen in different tissues as the inclusion of wheat flour increased. Modifications in intermediary metabolism revealed a decrease in amino acids catabolism coupled with a higher use of free glucose as carbohydrates rise up to 20%. However, this effect seems to be limited by the metabolic capacity of lobsters to use more than 20% of carbohydrates in diets. Lobsters were not able to tightly regulate α-amylase expression according to dietary carbohydrate level but exhibited a marked difference in secretion of this enzyme into the gut. Results are discussed to highlight the limitations to increasing carbohydrate utilization by lobsters. Further growout trials are needed to link the presented metabolic profiles with phenotypic outcomes.
PB PeerJ Inc.
SN 2167-8359
YR 2017
FD 2017
LK http://hdl.handle.net/10498/36652
UL http://hdl.handle.net/10498/36652
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026