RT journal article
T1 Bioprocess development for the production of poly(3-hydroxybutyrate) using the invasive macroalgae Rugulopteryx okamurae
A1 Agabo García, Cristina
A1 Sarafidou, Mirva
A1 Psaki, Olga
A1 Argeiti, Chrysanthi
A1 Blandino Garrido, Ana María
A1 Álvarez Gallego, Carlos José
A1 Romero García, Luis Isidoro
A1 Koutinas, Apostolis
A2 Ingeniería Química y Tecnología de Alimentos
K1 Rugulopteryx okamurae
K1 Solid-state fermentation
K1 Enzymatic hydrolysis
K1 Paraburkholderia sacchari
K1 Poly(3-hydroxybutyrate)
AB The brown macroalga Rugulopteryx okamurae was used to produce a sugar-rich hydrolysate,which served as a carbon source in Paraburkholderia sacchari DSM 17165 cultures for the productionof poly(3-hydroxybutyrate) (PHB). Biological pretreatment of R. okamurae using thefungal strains Aspergillus awamori and Aspergillus oryzae in solid-state fermentation (SSF) wasemployed to produce alginate lyase (24 IU/gbiomass) and protease (20 IU/gbiomass). The fermentedsolids were used for enzymatic hydrolysis in combination with a commercial cocktail of enzymescontaining cellulase and hemicellulase. SSF of the macroalgae for 72 h by A. oryzae, followed byenzymatic saccharification for 48 h, resulted in a total reducing sugars (TRS) concentration ofaround 120 mgTRS/gbiomass. Conducting a bioreactor batch fermentation on macroalgae-derivedhydrolysate for PHB production by P. sacchari, consuming 51 g/L of glucose, resulted in43.3 g/L of dry cell weight with 28.4 % PHB content (12.3 g/L PHB). This study demonstrated thefeasibility of exploiting macroalgae as a feedstock for PHB production.
PB Elsevier
SN 2352-1864
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36665
UL http://hdl.handle.net/10498/36665
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026