RT journal article
T1 Evolution of energy related metabolites in plasma from newborns with hypoxic-ischemic encephalopathy during hypothermia treatment
A1 Sánchez Illana, Ángel
A1 Núñez Ramiro, Antonio
A1 Cernada, María
A1 Parra Llorca, Anna
A1 Valverde, Eva
A1 Blanco, Dorotea
A1 Moral Pumarega, María Teresa
A1 Cabañas, Fernando
A1 Boix, Héctor
A1 Pavón, Antonio
A1 Chafanel, Mercedes
A1 Benavente Fernández, Isabel
A1 Tofe, Inés
A1 Loureiro, Begoña
A1 Fernández Lorenzo, José R.
A1 Fernández Colomer, Belén
A1 García Robles, Ana
A1 Kuligowski, Julia
A1 Vento, Máximo
A1 Cordeiro, M.
A1 Arriaga, M.
A1 Ureta Velasco, N.
A1 Caballero, M.A.
A1 Fernández, C.
A1 Castilla, Y.
A1 Ferreira, J.F.
A1 Lubián López, Simón Pedro
A1 Jaraba, P.
A1 López Heredia, J.
A2 Materno-Infantil y Radiología
AB Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying the evolution of several energy related key metabolites in newborns with HIE undergoing TH employing gas chromatography - mass spectrometry. The method was validated following stringent FDA requirements and applied to 194 samples from a subgroup of newborns with HIE (N = 61) enrolled in a multicenter clinical trial (HYPOTOP) for the determination of lactate, pyruvate, ketone bodies and several Krebs cycle metabolites at different sampling time points. The analysis of plasma samples from newborns with HIE revealed a decrease of lactate, pyruvate and β-hydroxybutyrate concentrations, whereas rising malate concentrations were observed. In healthy control newborns (N = 19) significantly lower levels of pyruvate and lactate were found in comparison to age-matched newborns with HIE undergoing TH, whereas acetoacetate and β-hydroxybutyrate levels were clearly increased. Access to a validated analytical method and a controlled cohort of newborns with HIE undergoing hypothermia treatment for the first time allowed the in-depth study of the evolution of key metabolites of metabolic junctions in this special population.
PB Nature Publishing Group
SN 2045-2322
YR 2017
FD 2017
LK http://hdl.handle.net/10498/36670
UL http://hdl.handle.net/10498/36670
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026