RT journal article
T1 Effect of an ozone pre-treatment on dark fermentation: Influence on the microbiota and pathogen removal
A1 Ibáñez López, María Eugenia
A1 Díaz Domínguez, Encarnación
A1 García Morales, José Luis
A2 Tecnologías del Medio Ambiente
K1 Bio-hidrógeno
K1 Ácidos Grasos Volátiles
K1 Ozono
K1 Fermentación Oscura
K1 Microbiota
K1 Patógenos
K1 Hydrogen production
K1 Population dynamics
K1 Hydrolytic-acidogenic bacteria
K1 Hydrolysis yield
K1 Acidic carbon
AB The aim of this study was to investigate the evolution of the microbial community and its relationship with hydrogenproduction, as well as pathogen reduction during thermophilic dark fermentation using sewage sludgeand wine vinasses, under ozone pre-treatment (PT) and non-pre-treated (Control) conditions. Microbial dynamicsanalysis revealed that PT increased the Eubacteria ratio and shifted the balance between Hydrolytic-Acidogenic Bacteria (HABs) and acetogens. PT enhanced bio-hydrogen production, achieving a 78 % increase inyield and a 90 % improvement in specific hydrogen production compared to control. In both conditions, Eubacteriapredominated over Archaea, with a higher proportion of Propionate-Utilizing Acetogens in comparison withButyrate-Utilizing Acetogens. Hydrolytic activity was 25 % higher in PT, correlating with the highest hydrogenproduction, reduced non-soluble carbon, and the most active HABs. PT also increased acidification yields by22 % and microbial activity by 80 %, enhancing hydrogen production primarily through elevated hydrolytic activity.This was associated with a 60 % rise in acid carbon, with acetic and butyric acids comprising 80 % of thetotal volatile fatty acids. Regarding pathogen reduction, PT reduced Escherichia coli concentrations in the feed byan order of magnitude. In conclusion, PT significantly improved bio-hydrogen production, microbial activity,and process efficiency, while facilitating effective pathogen reduction.
PB Elsevier
SN 1095-8630 ; 0301-4797
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36768
UL http://hdl.handle.net/10498/36768
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 09-nov-2026