RT journal article
T1 Ozonation Strategies to Maximize Bio-Hydrogen and Volatile Fatty Acid Production in Dark Co-Fermentation Process
A1 Ibáñez López, María Eugenia
A1 Díaz Domínguez, Encarnación
A1 García Morales, José Luis
A2 Tecnologías del Medio Ambiente
K1 Bio-hidrógeno
K1 Ácidos Grasos Volátiles
K1 Ozono
K1 Fermentación Oscura
K1 Biogaspre
K1 pre-treatment
K1 sludge
K1 thermophilic
K1 wine vinasse
AB Organic waste treatment through co-fermentation presents a promising approach for biogasproduction and the generation of valuable compounds. However, wastes like sewage sludge (SS)and wine vinasse (WV) have inherent limitations that hinder process efficiency. This study aimed to10 optimize the thermophilic dark co-fermentation (55°C) of SS and WV at a 50:50 volume ratio byemploying ozone pre-treatment to enhance bio-hydrogen and volatile fatty acid (VFA) production.Two ozonation strategies were evaluated: ozonation of the SS/WV mixture (SSO/WVO) and ozonationof SS followed by WV addition (SSO/WV), using an optimized ozone dose of 0.018 g O3/g TS0.Key parameters, including dissolved organic carbon (DOC), total acidity, total volatile fatty acids15 (TVFAs), and bio-hydrogen production, were analyzed. The findings revealed that the SSO/WVstrategy significantly increased DOC and TVFA yields, particularly short-chain fatty acids (SCFAs)and medium-chain fatty acids (MCFAs) such as butyric and valeric acids. This strategy resulted ina 370% increase in TVFAs and a 50% enhancement in bio-hydrogen production compared tountreated controls. In conclusion, ozone pre-treatment, especially the SSO/WV strategy, effectively20 enhanced VFA and bio-hydrogen yields during SS/WV co-fermentation. This approach providesa promising pathway to optimize the conversion of organic waste into high-value products, withnotable implications for agro-industrial waste management and bioenergy production.
PB Taylor & Francis
SN 0191-9512
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36774
UL http://hdl.handle.net/10498/36774
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 09-nov-2026