RT journal article
T1 Enhancing dark fermentative biohydrogen and VFA production via ozone pre-treatment
A1 Ibáñez López, María Eugenia
A1 Díaz Domínguez, Encarnación
A1 Fernandez-Morales, Francisco Jesus
A1 García Morales, José Luis
A2 Tecnologías del Medio Ambiente
K1 Sewage sludge
K1 Wine vinasse
K1 Ozone
K1 Particulate substrate hydrolysis
K1 Dark fermentation
AB This study investigates the effects of ozone pre-treatment on two types of organic wastes: secondary sludge (SS) and wine vinasse (WV). Ozone pre-treatment of SS, a semi-solid waste, significantly increased the Dissolved Organic Carbon (DOC) and Total Volatile Fatty Acids (TVFAs) through hydrolysis. Conversely, ozone pretreatment of WV, a liquid organic waste, reduced the availability of soluble biodegradable substrates and decreased the concentration of carboxylic acids with carbon chain length higher than 4. Based on these findings, the impact of ozonation on subsequent dark fermentation Biochemical Hydrogen Potential (BHP) was assessed for SS. The results, modeled for an ozone dose of 0.018 g O3/g TS, indicated a substantial enhancement in biohydrogen production by approximately 160% and VFA production by about 350%. In conclusion, ozone pretreatment shows significant potential to enhance dark fermentation of particulate substrates like secondary sludge, supporting sustainable waste valorization strategies.
PB Elseiver
SN 1873-2976
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36906
UL http://hdl.handle.net/10498/36906
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 09-nov-2026