RT journal article
T1 Development and Validation of an HPLC–DAD Method for the Quantification of Divanillin and the Main Compounds Present in Vanilla planifolia Jacks. ex Andrew
A1 Torres‑Zamudio, Yunuen Erandy
A1 Rodríguez-Jimenes, Guadalupe del Carmen
A1 Fernández Barbero, Gerardo
A1 Pérez-Silvia, Araceli
A2 Química Analítica
K1 Aromatic compounds
K1 Divanillin
K1 HPLC–DAD
K1 Phenols
K1 Validation
K1 Vanilla planifolia
AB This work presents a developed and validated high-performance liquid chromatography with a diode array detection (HPLC–DAD) method for the separation and quantification of divanillin and 8 compounds (p-hydroxybenzyl alcohol, vanillylalcohol, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, vanillic acid, vanillin, anisyl alcohol and anisic acid) present inVanilla planifolia Jacks. ex Andrews. Chromatographic separation was achieved in 15 min using A Zorbax Eclipse XDBC18column (250 mm × 4.6 mm i.d., 5 μm particle size). Gradient elution was performed using a solvent mixture of water,methanol and acidified water (10–2 M H3PO4),at a flow rate of 2.25 mL/min with detection at 230, 254 and 280 nm. Themethod was comprehensively validated according to the International Conference of Harmonization (ICH) Q2 (R1) guidelines.The method was linear in 0.1–200 mg/L concentration range with coefficient of determination (r2) higher than 0.99.The percentage recovery ranged from 98.04 to 101.83% with a relative standard deviation of less than 2%, confirming themethod's accuracy and precision for the analysis of nine compounds. Existing methods for analyzing aromatic compounds invanilla, such as HPLC–DAD, GC–MS, and NMR, often overlook divanillin or lack validated protocols for its quantificationalongside other compounds. This study presents a significant advancement by developing a robust, validated HPLC–DADmethod that enables the simultaneous quantification of divanillin, vanillin, and other key phenolic compounds with evidenceof divanillin presence in all analyzed samples in cured pods of Vanilla planifolia Jacks. ex Andrews, with concentrationsranging from 0.002 to 0.02 g/100g dry weight.
PB Springer Nature
SN 1936-9751 ; 1936-976X
YR 2025
FD 2025
LK http://hdl.handle.net/10498/36970
UL http://hdl.handle.net/10498/36970
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 09-nov-2026