RT journal article
T1 Use of Trichoderma, Aspergillus, and Rhizopus Fungi for the Biological Production of Hydrolytic Enzymes and Uronic Acids from Sargassum Biomas
A1 Agabo García, Cristina
A1 Cahyanto, M.N.
A1 Setyaningsih, W.
A1 Romero García, Luis Isidoro
A1 Álvarez Gallego, Carlos José
A1 Blandino Garrido, Ana María
A2 Ingeniería Química y Tecnología de Alimentos
K1 solid-state fermentation
K1 Sargassum sp.
K1 alginate lyase
K1 cellulase
K1 laminarinase
K1 uronic acids
K1 total phenolic compounds
AB The objective of this study was the evaluation of fungal solid-state fermentation (SSF) for the production of alginate lyase and extraction of uronic acids from Sargassum sp. For this purpose, the fungi Trichoderma asperellum, Aspergillus oryzae, and Rhizopus oryzae were applied (alone or combined) to Sargassum sp. biomass through SSF (107 spores gbiomass−1, 30 °C, and 7 days of treatment). In general, individual SSF with all three fungi degraded the biomass, achieving a marked synergy in the production of cellulase, laminarinase, and alginate lyase activities (especially for the last one). Trichoderma was the most efficient species in producing laminarinase, whereas Rhizophus was the best option for producing alginate lyase. However, when dual combinations were tested, the maximal values of alginate lyase activities were reached (13.4 ± 0.2 IU gbiomass−1 for Aspergillus oryzae and Rhizopus oryzae). Remarkably, uronic acids were the main monomeric units from algal biomass solubilization, achieving a maximum yield of 14.4 mguronic gbiomass−1, with the A + R condition being a feasible, eco-friendly alternative to chemical extraction of this monomer. Additionally, the application of all the fungal pretreatments drastically decreased the total phenolic content (TPC) in the biomass from 369 mg L−1 to values around 44–84 mg L−1, minimizing the inhibition for possible subsequent biological processes in which the residual solid can be used.
PB MDPI
SN 2311-5637
YR 2025
FD 2025
LK http://hdl.handle.net/10498/37457
UL http://hdl.handle.net/10498/37457
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026