RT journal article
T1 Modulation of PUFA and PUA production in coastal diatoms by varying light and phosphate availability
A1 Hernanz Torrijos, María
A1 Bartual Magro, Ana
A1 Ortega Agüera, María Jesús
A2 Biología
A2 Química Orgánica
K1 Polyunsaturated fatty acids
K1 Polyunsaturated aldehydes
K1 Phosphate availability
K1 Light quality
K1 Biotechnological optimization
K1 Culture condition
AB As climate change intensifies and resource sustainability becomes crucial, diatoms represent a promising sourceof bioactive compounds. Among them, polyunsaturated fatty acids (PUFA) stand out for their essential biologicalroles and high value in fields such as medicine, aquaculture and bioenergy. However, in several diatom species,PUFA productivity can be reduced due to their enzymatic conversion into polyunsaturated aldehydes (PUA),compromising their biotechnological potential. To evaluate the true potential of PUA-producing diatoms as PUFAsources, we introduced a transformation ratio metric that estimates the proportion of PUFA availability after PUAproduction. This metric was applied to three coastal diatoms (Thalassiosira minima, Skeletonema pseudocostatum,and Cyclotella cryptica) cultured under different phosphate availability and light conditions. A selection frame-work identified T. minima as unsuitable due to high PUA production relative to PUFA levels. S. pseudocostatumand C. cryptica were further analyzed, showing different optimal culture conditions. In S. pseudocostatum, highphosphate and cool white light conditions reduced PUA production, while warm light and continuous photo-periods increased cell stress and PUA production, raising the transformation ratio to the maximum detected(21.93 ± 5.10 %). In contrast, C. cryptica showed stable PUA production across treatments and enhanced PUFAavailability under low phosphate and cool light. Under these conditions, PUFA concentration reached 1181.81 ±209.11 fmol cell 1, the highest among all species and condition tested. This study provides a biotechnologicalscreening criterion for PUA-producing diatom optimizing culture conditions
PB Elsevier
SN 2211-9264
YR 2025
FD 2025-10-08
LK http://hdl.handle.net/10498/37602
UL http://hdl.handle.net/10498/37602
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026