RT journal article
T1 Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils
A1 Aguiar del Río, Juan Francisco
A1 Liberal González, Isabel María
A1 Muñoz Rueda, María Luisa
A1 Cantos-Villar, Emma
A1 Puertas, Belén
A1 Archidona-Yuste, Antonio
A1 Casimiro-Soriguer Camacho, Ramón
A1 Coletta, Martina
A1 D’Alessandro, Aldo
A1 González Robles, Ana
A1 Gramaje, David
A1 Renard, Delphine
A1 Roy, Mélanie
A1 La Terza, Antonietta
A1 Tarifa Murcia, Rubén
A1 Vacher, Corinne
A1 Veen, G.F.(Ciska)
A1 Ochoa Hueso, Raúl
A2 Biología
K1 Solid-phase fermentation
K1 Biofertilizer
K1 Inoculum
K1 Soil microorganisms
K1 Enzyme activity
K1 Rhizosphere
AB Achieving the sustainability of modern agriculture will require, among other actions, an improvement of the soil and its associated microbiota. One way to achieve this is through the inoculation with beneficial soil microbial communities. In this study, we used solid-phase fermentations to produce 25 distinct microbial inoculants based on complex communities obtained from the rhizosphere of 23 European vineyards. For this purpose, we mixed 0.1 g of donor rhizosphere soil and 25 g of ground and sterilized growing substrate composed of winemaking byproducts in 15-cm Petri dishes. Aerobic fermentations were carried out for a period of 56 days and the activity of microbial enzymes linked to the biogeochemical cycling of carbon, nitrogen, phosphorus, and sulfur was evaluated every two weeks. We then carried out a common garden experiment where the inoculants were tested in pots containing vineyard soil and planted with Tempranillo vines. During the fermentation, the enzyme activity of the inoculants evolved from no activity to high-activity values. Carbon- and phosphorus-linked enzymes tended to show higher activity after 14 days of incubation and then decreased or remained constant, while nitrogen-linked enzymes tended to show their highest values after 28 days of incubation. Despite these general patterns, inoculants developed from different rhizosphere communities followed different trajectories in terms of activity. In addition, we observed significant relationships between the enzyme activity of donor rhizosphere soils and the enzyme activity of inoculants, especially after 28 days of incubation. We also found a significant relationship between the enzyme activity of the inoculants and the enzyme activity measured in the soil of pots containing vines. Our results suggest the possibility of predicting the metabolic potential of the inoculants from the metabolic potential of their donor soil sample, as well as the possibility of transferring these metabolic capabilities to soils, with likely applications for the regeneration of the functioning of vineyards.
PB Elsevier
SN 2452-2198
YR 2025
FD 2025-02-20
LK http://hdl.handle.net/10498/37858
UL http://hdl.handle.net/10498/37858
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026