RT journal article T1 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation A1 León Marcos, Ludisbel A1 Montes Herrera, Antonio A1 Valor López, Diego A1 García Casas, Ignacio A1 Machado, Noelia Daiana A1 Pereyra López, Clara María A2 Ingeniería Química y Tecnología de Alimentos K1 Poly(3-hydroxi-butyrate-co-3-hydroxyvalerate) K1 Supercritical Solvent Impregnation K1 Mangifera indica L. leaves K1 Active food packaging AB The impregnation of the polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with mango leaves extract (MLE) was carried out using the Supercritical Solvent Impregnation method. The impregnation loads, antioxidant compounds and physical properties of impregnated polymers were investigated with the variation of operating parameters (temperature (35 and 55 ◦ C), pressure (10, 20 and 30MPa) and depressurization rate (0.1 and 5MPa/min)). Furthermore, the influence of varying impregnation times (1, 3, 6, 10, 24h) with 30MPa, 35 ◦ Cand5MPa/minwasalsoinvestigated and the antimicrobial capacity (AC) was determined. The results showed a decrease of the impregnation loads, antioxidant and antimicrobial capacities with increasing impregnation time. At 30MPa, 35 ºC and 5MPa/min with an impregnation time of 1h, impregnation loads and antioxidant capacities of 2.82 ±0.04mg MLE/100mg PHB-HV and 2.77±0.10mg AC/100mg PHB-HV, respectively, and a growth inhibition percentage against Staphylococcus aureus and Escherichia coli bacteria greater than 60 % were obtained. PB Elsevier SN 2212-9820 YR 2025 FD 2025 LK http://hdl.handle.net/10498/37890 UL http://hdl.handle.net/10498/37890 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 21-sep-2026