RT book part
T1 Fish embryonic stem cells as tools for chronobiological and endocrinological studies
T2 Células madre embrionarias de peces como herramientas para estudios cronobiológicos y endocrinológicos
A1 Vergés Castillo, Alba
A1 Pendón Meléndez, Carlos
A1 Muñoz Cueto, José Antonio
A1 Martín Robles, Agueda Jimena
A2 Biología
A2 BiomedicinaBiotecnología y Salud Pública
K1 Sparus aurata
K1 Embryonic stem cells
K1 Circadian rhythms
K1 Fish chronobiology
K1 Clock genes
K1 Cell line
AB Circadian rhythms exist in many biochemical, physiological and cellular processes, including hormonal synthesis and/or secretion. These rhythms are driven by endogenous clocks that are ultimately entrained by environmental factors, being the light-dark cycle (LD) the most important synchronizer. Fish circadian rhythms appear sustained by cellautonomous mechanisms, but unfortunately, fish cellular  clocks have been poorly studied in marine species. An important bottleneck in fish research is the supply and maintenance of embryos, larvae, juvenile and adult specimens. In this context, cell lines constitute powerful and alternative tools with important applications in fish chronobiology and endocrinology. In this study, we have used a recently developed monoclonal embryonic cell line derived from the blastula stage of gilthead seabream embryos (SAEC-H7) to analyse the oscillation of central clock components and the response to different photoregimes. Our results showed rhythmic expression of key clock genes in embryonic cells, which were able to re-entrain to a light cycle inversion. Rhythmic expression of some core clock genes persisted under constant light and dark conditions after several days, but with decreased mesors and amplitudes, reinforcing the circadian and endogenous nature of the cellular rhythms. These results indicate that SAEC-H7 contain a functional molecular clock entrained by light, representing an important tool for chronobiological and endocrinological studies in fish.
PB Pedro M. Guerreiro e João C. R. Cardoso
SN 978-989-9023-41-3 (printed version)
YR 2021
FD 2021
LK http://hdl.handle.net/10498/38449
UL http://hdl.handle.net/10498/38449
LA eng
NO Proceedings from communications presented at the XII Conference of the Iberian Association for Comparative Endocrinology (AIEC), held from the 26th to the 28th of September 2019 at the University of Algarve, Faro, Portugal.
NO ASOCIACIÓN IBÉRICA DE ENDOCRINOLOGÍA COMPARADA (AIEC)Universidade do AlgarveCentro de Ciencias do Mar (CCMAR)Fundaçáo para a Ciéncia e a Tecnología
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026