RT journal article
T1 Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts
A1 Galan Rodriguez, Beatriz
A1 Marco Delgado, Ángel José del
A1 Flores, Juan Antonio
A1 Ramiro Fuentes, Susana
A1 González Aparicio, R.
A1 Tunez, I.
A1 Tasset, I.
A1 Fernandez Espejo, Emilio
A2 BiomedicinaBiotecnología y Salud Pública
K1 Extra-adrenal
K1 Graft
K1 Parkinson
K1 GDNF
K1 TGFβ1
K1 Trophic
K1 Regeneration
K1 Zuckerkandl's paraganglion
AB The objective was to discern the neuroregenerative effect of grafts of extra-adrenal cells of the Zuckerkandl's paraganglion (ZP) in the nigrostriatal circuit, by using the retrograde model of parkinsonism in rats. The antiparkinsonian efficacy of two types of grafting procedures wasstudied (cell aggregates vs. dispersed cells), and GDNF and TGFβ1 (dopaminotrophic factors) as well as dopamine presence in extraadrenal tissue was analyzed. Extra-adrenal chromaffin cells are noradrenergics, tissue dopamine is low, and they express both GDNF and TGFβ1. Grafts of cell aggregates, not of dispersed cells, exerted a trophic regeneration of the host striatum, leading to amelioration of motor deficits. Sprouting of spared dopaminergic fibers within the striatum, reduction of dopamine axon degeneration, and/or enhanced phenotypic expression of TH would explain striatal regeneration. Grafted cells as aggregates showed a better survival rate than dispersed cells, and they express higher levels of GDNF. Higher survivability and GDNFcontenttogether with the neurorestorative and dopaminotrophic actionof bothGDNFandTGFβ1couldaccountforstriatalrecoveryand functional amelioration after grafting ZP cell aggregates. Finally, nigral degeneration and partial degeneration of ventral tegmental area were not precluded after transplantation, indicating that the trophic effect of grafts was local within the host striatum.
PB Elsevier
SN 0969-9961
YR 2008
FD 2008
LK http://hdl.handle.net/10498/38604
UL http://hdl.handle.net/10498/38604
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026