RT journal article
T1 Proteomics-Based Approaches to Decipher the Molecular Strategies of Botrytis cinerea: A Review
A1 Coste, O.B.N.
A1 Escobar Niño, Almudena
A1 Fernández Acero, Francisco Javier
A2 BiomedicinaBiotecnología y Salud Pública
K1 Botrytis cinerea
K1 extracellular vesicles
K1 fungal virulence
K1 fungicide resistance
K1 molecular mechanisms
K1 pathogenesis
K1 plant defense;
K1 plant-pathogen interaction
K1 proteomics
K1 secretome
AB Botrytis cinerea is a highly versatile pathogenic fungus, causing significant damage across a wide range of plant species. A central focus of this review is the recent advances made through proteomics, an advanced molecular tool, in understanding the mechanisms of B. cinerea infection. Recent advances in mass spectrometry-based proteomics—including LC-MS/MS, iTRAQ, MALDI-TOF, and surface shaving—have enabled the in-depth characterization of B. cinerea subproteomes such as the secretome, surfactome, phosphoproteome, and extracellular vesicles, revealing condition-specific pathogenic mechanisms. Notably, in under a decade, the proportion of predicted proteins experimentally identified has increased from 10% to 52%, reflecting the rapid progress in proteomic capabilities. We explore how proteomic studies have significantly enhanced our knowledge of the fungus secretome and the role of extracellular vesicles (EVs), which play key roles in pathogenesis, by identifying secreted proteins—such as pH-responsive elements—that may serve as biomarkers and therapeutic targets. These technologies have also uncovered fine regulatory mechanisms across multiple levels of the fungal proteome, including post-translational modifications (PTMs), the phosphomembranome, and the surfactome, providing a more integrated view of its infection strategy. Moreover, proteomic approaches have contributed to a better understanding of host–pathogen interactions, including aspects of the plant’s defensive responses. Furthermore, this review discusses how proteomic data have helped to identify metabolic pathways affected by novel, more environmentally friendly antifungal compounds. A further update on the advances achieved in the field of proteomics discovery for the organism under consideration is provided in this paper, along with a perspective on emerging tools and future developments expected to accelerate research and improve targeted intervention strategies.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2309-608X
YR 2025
FD 2025
LK http://hdl.handle.net/10498/38655
UL http://hdl.handle.net/10498/38655
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026