RT journal article
T1 Analytical determination of the reducing and stabilization agents present in different Zostera noltii extracts used for the biosynthesis of gold nanoparticles
A1 Zarzuela Sánchez, Rafael
A1 Luna Aguilera, Manuel Jesús
A1 Gil Montero, María Luisa Almoraima
A1 Ortega Agüera, María Jesús
A1 Palacios Santander, José María
A1 Naranjo Rodríguez, Ignacio
A1 Delgado Jaén, Juan José
A1 Cubillana Aguilera, Laura
A2 Ciencia de los Materiales e Ingeniería Metalúrgica y Química Inorgánica
A2 Química Analítica
A2 Química Física
A2 Química Orgánica
AB ABSTRACTThe objective of this work was to ascertain the nature of the components responsible for the reducing and stabilizing properties of Zostera noltii extracts that lead to gold nanoparticle formation using chemical techniques of analysis. In order to achieve this aim, we try the synthesis of AuNPs with three different extracts from plants collected in the Bay of Cádiz (Spain). The n-butanol extract produced the best results. Taking this into account, four fractions were isolated by Sephadex LH-20 column chromatography from this extract and we studied their activity. The chemical study of these fractions led to the isolation of several flavone sulfates and these were identified as the species' responsible for the formation and stabilization of the AuNPs. Flavone sulfates were purified by high performance liquid chromatography and the structures were established by means of spec- troscopic methods nuclear magnetic resonance and mass spectroscopy. AuNPs have an average lifetime of about 16 weeks. Additionally, the morphology and crystalline phase of the gold nanoparticles were characterized by transmission electron microscopy. The composition of the nanoparticles was evaluated by electron diffraction and energy dispersive X-ray spectroscopy. An 88% of the gold nanoparticles has a diameter in the range 20–35 nm, with an average size of 26 ± 2 nm.1.
PB Elsevier
SN 1873-2682
YR 2018
FD 2018
LK http://hdl.handle.net/10498/38781
UL http://hdl.handle.net/10498/38781
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026