RT journal article T1 Optimizing Biomethane Production from Industrial Pig Slurry and Wine Vinasse: A Mathematical Approach A1 Cañadas Marín, Belén A1 Fernández Rodríguez, Juana A1 Solera del Río, María del Rosario A1 Pérez García, Montserrat A2 Tecnologías del Medio Ambiente K1 biomethane K1 Biochemical Methane Potential (BMP) K1 anaerobic co-digestion (AcoD) K1 Design of Experiments (DoE) K1 Box–Behnken design (BBD) AB Pig slurry (PS) and wine vinasse (WV) pose environmental risks if not properlymanaged. Their composition makes them suitable for anaerobic co-digestion (AcoD),enhancing biomethane production and improving organic matter degradation efficiency.This research applies an innovative Design of Experiments (DoE) approach—specificallythe Box–Behnken design (BBD)—to systematically optimize the AcoD process, surpassingtraditional single-factor methods by efficiently evaluating the interactions. Variables suchas temperature (35 ◦C, 52.5 ◦C, 70 ◦C), substrate ratio (25PS:75WV, 50PS:50WV, 75PS:25WV)and pH (7, 7.5, 8) were tested using a Box–Behnken design which studied the correlationsbetween the experimental data and the model. In fact, the results showed that temperature,ratio, and their interaction significantly influenced biomethane production, being the pH thefactor with the least influence on the response. Optimal conditions—pH of 8, temperatureof 35 ◦C and a 50:50 substrate ratio—achieved a biomethane yield of 487.94 CH4/gVS(Volatile Solids). These results demonstrate the effectiveness of the DoE methodology inmaximizing biomethane production and represent a significant advancement in valorizingwastes from pig farms and wineries, promoting a circular and sustainable economy. PB MDPI SN 2305-7084 YR 2025 FD 2025-06-03 LK http://hdl.handle.net/10498/38871 UL http://hdl.handle.net/10498/38871 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 07-nov-2026