RT journal article
T1 Vasculogenic potential of adipose tissue derived stem cells from patients with chronic spinal cord injury and pressure injuries
A1 Santos de la Mata, Ángela
A1 Esteban, Pedro F.
A1 Martínez Torija, Mario
A1 Paniagua Torija, Beatriz
A1 Espino-Rodríguez, Francisco Javier
A1 Beltrán Camacho, Lucía
A1 Camacho Toledano, Celia
A1 Alcobendas Maestro, Mónica
A1 García García, Fernando
A1 Molina Holgado, Eduardo
A1 Durán Ruiz, María del Carmen
A1 Melero Martin, Juan M.
A1 Moreno Luna, Rafael
A2 BiomedicinaBiotecnología y Salud Pública
K1 Spinal cord injury
K1 Pressure injury
K1 Adipose tissue
K1 Angiogenesis
K1 Vasculogenesis
K1 ASCs
K1 MSCs
K1 ECFCs
AB Limited vascularization and ischemia are major contributors to the chronicity of wounds, such as ulcers and traumaticinjuries, which impose significant medical, social, and economic burdens. These challenges are particularly pronounced inpatients with spinal cord injury (SCI), a disabling condition associated with vascular dysfunction, infections, and impairedperipheral circulation, complicating the treatment of pressure injuries (PIs) and the success of reconstructive procedureslike grafts and flaps. Regenerative medicine aims to address these issues by identifying effective cellular therapies torestore vascular beds. Among these, cells from the stromal vascular fraction (SVF) of adipose tissue (AT) are promisingdue to their abundance of angiogenic and vasculogenic cells, including mesenchymal stem cells (MSCs) and endothelialcolony-forming cells (ECFCs). This study evaluated the vasculogenic potential of AT-derived cellular fractions isolated viaenzymatic digestion of white adipose tissue (WAT). We compared adipose-derived stem cells (ASCs) cultured from SVFwith a combination of ECFCs and MSCs, expanded separately and transplanted in a 40:60 ratio. Results showed that whileASCs promote angiogenesis and vasculogenesis, the ECFC/MSC combination is superior, consistently forming perfusedvascular beds in subcutaneous implants in nude mice. Furthermore, ECFCs and MSCs extracted from small amounts ofWAT in SCI patients with PIs demonstrated similar functionality and vasculogenic potential to cells from healthy controls.These findings highlight the potential of AT-derived ECFCs and MSCs in autologous cell therapies, offering a promisingavenue for advancing vascular regeneration in patients with SCI.
PB Springer
SN 1573-7209
YR 2025
FD 2025-09-10
LK http://hdl.handle.net/10498/39039
UL http://hdl.handle.net/10498/39039
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026