RT journal article
T1 Ultrasound-Assisted Extraction of Patin Fish Oil: Optimization Using Response Surface Methodology – Box Behnken Design
A1 Putri, Anggita Rosiana
A1 Setyaningsih, Widiastuti
A1 Palma Lovillo, Miguel
A1 Carrera Fernández, Ceferino Adrián
A1 Rohman, Abdul
A1 Riyanto, Sugeng
A1 Jang, Young Pyo
A2 Química Analítica
K1 PFO
K1 optimization
K1 ultrasound-assisted extraction
K1 esponse surface methodology
K1 Box-Behnken design
AB The oil extracted from Pangasius micronemus, commonly known as patinfish, is highly regarded for its omega-3 and omega-6 fatty acids content, which offernumerous health benefits. Producing high-quality patin fish oil (PFO) rich in theseessential fatty acids requires an optimized extraction process. This study utilizedultrasound-assisted extraction (UAE) combined with response surface methodology – BoxBehnken Design (RSM-BBD) to optimize the extraction of PFO. Five variables wereexamined: temperature (30–60 °C), solvent composition (n-hexane in isopropanol, 30–90%), amplitude (30–90%), solvent-to-sample ratio (10:1–20:1), and cycle duration (0.2–0.8 s−1). The analysis identified solvent composition, amplitude, and solvent-to-sampleratio as significant factors influencing the response value (p < 0.005). Optimal UAEconditions were achieved at a temperature of 59 °C, solvent composition of 42%,amplitude of 41%, solvent-to-sample ratio of 20:1, cycle duration of 0.8 s−1, and anextraction time of 25 min. Fatty acid profiling revealed that PFO extracted using UAEcontained omega-3 fatty acids, including α-linolenic acid (ALA) at 1.36% andeicosapentaenoic acid (EPA) at 0.46%, as well as omega-6 fatty acids, namely linoleic acid(LA) at 19.06% and arachidonic acid (AA) at 0.85%. These results demonstrate theefficiency of UAE in extracting high-quality PFO.
PB Gadjah Mada University
SN 2460-1578
YR 2025
FD 2025
LK http://hdl.handle.net/10498/39305
UL http://hdl.handle.net/10498/39305
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026