RT journal article
T1 A prospective survey of Aspergillus spp. in respiratory tract samples: Species identification and susceptibility patterns
A1 Castro, Carmen
A1 Galán Sánchez, Fátima
A1 Linares, Mª José
A1 Tejero, Rocío
A1 Ruiz, Maite
A1 Serrano, Mª Luisa
A1 Rodríguez Iglesias, Manuel Antonio
A1 Martín-Mazuelos, Estrella
A2 BiomedicinaBiotecnología y Salud Pública
K1 Aspergillus spp
K1 Cryptic species
K1 Hospitalized patients
K1 Respiratory tract samples
AB We analyzed the species distribution and susceptibility patterns of 433 strains of Aspergillus spp. isolated from respiratory samples of 419 in-patients included in multicenter prospective study (FUNGAE-IFI) between July 2014 and October 2015. Identification was carried out by conventional methods at each participating center and by molecular sequencing of a portion of the β-tubulin gene at one of the centers. In vitro susceptibility was evaluated by broth microdilution methods and using the E-test (for cryptic species). Species identified included 249 A. fumigatus sensu stricto, 60 A. terreus sensu stricto, 47 A. flavus sensu stricto, 44 A. tubingensis, 18 A. niger sensu stricto , five A. nidulans sensu stricto, three A. tamarii, two A. calidoustus, two A. carneus, one A. acuelatus, one A. carbonarius, and one A. sydowii. Cryptic species were found in 12.5% of isolates (n = 54). The frequency of non-wild-type isolates for amphotericin B was 3.4% (n = 15) of the isolates tested and for azoles 3% (n = 10). None of the Aspergillus spp. were non-wild type to echinocandins. Of the 54 cryptic species only two strains were non-wild-type strains by microdilution method (3.7%) (two A. tubingensis, one to amphotericin B and another one to voriconazole) and by E-test method five strains of A. tubingensis showed high minimal inhibitory concentration (MIC) to amphotericin B (11.4%) and five to azoles (12.1%), one A. calidoustus strain showed high MICs for three azoles (50%), A. carneus to itraconazole (100%) and A. sydowii to amphotericin B and itraconazole (100%). These results provide relevant information on susceptibility patterns, frequency, and epidemiology of species involved in respiratory tract samples and of the incidence of recently described cryptic species.
PB Oxford University Press
SN 1460-2709
YR 2019
FD 2019
LK http://hdl.handle.net/10498/40224
UL http://hdl.handle.net/10498/40224
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026