RT journal article
T1 Molecular characterization of consecutive isolates of OXA-48-producing Klebsiella pneumoniae: changes in the virulome using next-generation sequencing (NGS)
A1 Rodríguez Pallares, Salud
A1 Mateo Vargas, M. Alejandra
A1 Rodríguez Iglesias, Manuel Antonio
A1 Galán Sánchez, Fátima
A2 BiomedicinaBiotecnología y Salud Pública
K1 Klebsiella pneumoniae
K1 OXA-48
K1 Virulence factor
K1 Multi-locus sequence typing
K1 Antimicrobial resistance
AB Little is known about the clonality of consecutive OXA-48 producing-Klebsiella pneumoniae isolates fromthe same patient and the possibility of changes in their virulomes over time. We studied the molecularcharacteristics of twenty OXA-48-producing K. pneumoniae consecutive isolates from six patients usingwhole-genome sequencing. The genomes were screened for antimicrobial resistance and virulence factor genes and for replicon groups. MLST and SNPs analysis was performed.MLST analysis found 3 STs: ST11 (n ¼ 13; 65.0%); ST4975 (n ¼ 5, 25.0%); ST307 (n ¼ 2; 10.0%). AcrAb efflux pump, siderophore enterobactin and rcsAB capsule synthesis regulator were detected in all sequenced isolates. The regulator of mucoid phenotype A (rmpA) and rmpA2 were not detected. Isolatesalso carried type 3 fimbriae (n ¼ 19; 95.0%), yersiniabactin (n ¼ 15; 75.0%) and type 1 fimbriae (7; 35.0%).Type 3 fimbriae and yersiniabactin were lost and recovered in consecutive isolates of two patients, probably acquired by horizontal gene transfer. Our findings reveal that recurrent infections are due to the same isolate, with an average of 2.69 SNPs per month, with different virulence profiles, and that the acquisition of virulence factor genes over time is possible.
PB Elsevier
SN 1769-714X
YR 2023
FD 2023
LK http://hdl.handle.net/10498/40227
UL http://hdl.handle.net/10498/40227
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026