RT journal article
T1 Study of grapevine endophytes’ interaction with the host and their potential as biocontrol agents as sustainable alternative to agrochemicals
A1 Pizzi, Simona
A1 Di Canito, Alessandra
A1 Palencia Mulero, Elena
A1 De Oliveira Nobre Bezerra, Carmen Cris
A1 Ricciardi, Valentina
A1 Maddalena, Giuliana
A1 Maluleke, Evelyn
A1 Carmine Foschino, Roberto
A1 Toffolatti, Silvia Laura
A1 Fracassetti, Daniela
A1 De Lorenzis, Gabriella
A1 Cordero Bueso, Gustavo Adolfo
A1 Setati, Mathabatha Evodia
A1 Vigentini, Ileana
A2 BiomedicinaBiotecnología y Salud Pública
K1 Aureobasidium pullulans
K1 Bacillus velezensis
K1 Botrytis cinerea
K1 elicitation
K1 host interaction
K1 sustainability
AB In sustainable vineyard management, biocontrol agents (BCAs) offer a promising alternative to chemical pesticides. This study focuses on characterizing endophytes isolated from grapevines in different regions of northern Italy for (i) their effectiveness against Botrytis cinerea through in vitro tests and (ii) their capability to survive in the presence of copper and other commercial fungicides with the final goal of investigating their possible future application for an integrated approach in vineyard. Aureobasidium pullulans strains ED203, ED206, ED217, and ED221 demonstrated significant antagonistic activity toward B. cinerea, while Bacillus velezensis ED163 showed the highest levels of pathogen inhibition. Volatile organic compounds (VOCs) analysis identified three distinct microbial clusters based on BCAs volatile profiles, highlighting the production of antifungal compounds such as alcohols, esters, and terpenes to counteract the pathogen growth. The investigated BCAs showed tolerance to high concentrations of copper (up to 100 mg/L) and the fungicide SWITCH (1 g/L), but they were sensitive to a standard oenological dose of sulfur dioxide (50 mg/L). Finally, in vivo tests on grapevine leaves confirmed the ability of B. velezensis and A. pullulans strains ED203 and ED221 candidates to effectively reduce the mycelium infection on plant tissue standing out as strong candidates for pest management. The interaction between BCAs with the host was confirmed through leaves cell culture use, highlighting the capability of the best candidate selected in the study to modulate plant response in presence of pathogen.
PB Frontiers Media SA
SN 1664302X
YR 2026
FD 2026
LK http://hdl.handle.net/10498/40541
UL http://hdl.handle.net/10498/40541
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026