• español
    • English
  • Login
  • English 
    • español
    • English

UniversidaddeCádiz

Área de Biblioteca, Archivo y Publicaciones
Communities and Collections
View Item 
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Bio-guided optimization of the ultrasound-assisted extraction of compounds from Annona glabra L. leaves using the etiolated wheat coleoptile bioassay

Thumbnail
Identificadores

URI: http://hdl.handle.net/10498/20302

DOI: 10.1016/j.ultsonch.2014.01.024

Files
2014-Bio-guided optimization of the ultrasound-assisted extraction of compounds from Annona glabra L. leaves using the etiolated wheat coleoptile bioassay.pdf (671.7Kb)
Statistics
View statistics
Metrics and citations
 
Share
Export
Export reference to MendeleyRefworksEndNoteBibTexRIS
Metadata
Show full item record
Author/s
Matsumoto, Sadao; Valera Montoya, Rosa María; Palma Lovillo, MiguelAuthority UCA; González Molinillo, José MaríaAuthority UCA; Lima, Inês S.; García Barroso, Carmelo; Macías Domínguez, Francisco AntonioAuthority UCA
Date
2014-02
Department
Química Orgánica
Source
Ultrasonics Sonochemistry 21 (2014) 1578–1584
Abstract
A bio-guided optimization of the extraction of bioactive components from Annona glabra leaves has been developed using the etiolated wheat coleoptile bioassay as the control method. The optimization of an ultrasound-assisted extraction of bioactive compounds using allelopathy results as target values has been carried out for the first time. A two-level fractional factorial experimental design was applied to optimize the ultrasound-assisted extraction. The solvent was the extraction variable that had the most marked effect on the resulting bioactivity of the extracts in the etiolated wheat coleoptile bioassay. Extraction time, extraction temperature and the size of the ultrasonic probe also influenced the bioactivity of the extracts. A larger scale extraction was carried out in the next step in the allelopathic study, i.e., the isolation of compounds from the bioactive extract and chemical characterization by spectroscopic techniques, including NMR. Eight compounds were isolated and identified from the active extracts, namely two steroids (β-sistosterol and stigmasterol), five diterpenes with the kaurane skeleton (ent-kaur-16-en-19-oic acid, ent-19-methoxy-19-oxokauran-17-oic acid, annoglabasin B, ent-17-hydroxykaur-15-en-19-oic acid and ent-15β,16β-epoxy-17-hydroxy-kauran-19-oic acid) and the acetogenin asimicin. The most active compound was annoglabasin B, which showed inhibition with values of -95% at 10-3 M, -87% at 5 - 10-4 M and greater than -70% at 10-4 M in the etiolated wheat coleoptile bioassay.
Subjects
bio-guided extraction; ultrasound-assisted extraction; annona glabra; allelopathic activity
Collections
  • Artículos Científicos [4201]
  • Artículos Científicos INBIO [242]
  • Articulos Científicos Quim. Org. [158]
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Browse

All of RODINCommunities and CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics

Información adicional

AboutDeposit in RODINPoliciesGuidelinesRightsLinksStatisticsNewsFrequently Asked Questions

RODIN is available through

OpenAIREOAIsterRecolectaHispanaEuropeanaBaseDARTOATDGoogle Academic

Related links

Sherpa/RomeoDulcineaROAROpenDOARCreative CommonsORCID

RODIN está gestionado por el Área de Biblioteca, Archivo y Publicaciones de la Universidad de Cádiz

Contact informationSuggestionsUser Support