How Meaningful Are Minor Details in the Generation of Nanomodified Electrochemical Enzyme Biosensors? Exploring the Scenario with Sinusoidal Approaches

Abstract

In this work, a screening of Sonogel-Carbon (SNGC) electrodes modified with nanomaterials (carbon nanotubes and gold nanoparticles) and the study of their effect on the electrochemical performance of sinusoidal voltage (SV) and current (SC)-based biosensors are reported. Surface modification was achieved by drop-casting and electrodeposition methodologies. Within the strategies used, SV and SC, recently exploited procedures, were used to electrodeposit simultaneously a poly 3,4-ethylenedioxythiophene (PEDOT)-tyrosinase layer and the corresponding nanostructured material. Dopamine was selected as a benchmark analyte to evaluate the analytical performance of the different (bio)sensors obtained in terms of relevant figures of merit, such as sensitivity, limits of detection and quantitation, and accuracy, among others. A discussion about the pros and cons between the type of modification and the methods employed is also presented. Briefly, SC based sensors offered excellent quality analytical parameters and lower dispersion of the results. They were employed for more specific electrochemical studies, including interferences assays and the determination of DA in real samples, obtaining good recoveries (101-110.6%). The biosensor modified with gold nanoparticles (AuNPs) (drop-casting method) and SC-electrodeposited showed the best figures of merit: R-2 = 0.999; sensitivity = -4.92 x 10(-9) A.mu M-1; RSDsensitivity = 1.60%; LOD = 5.56 mu M; RSDLOD = 6.10%; and LOQ = 18.53 mu M.