• español
    • English
  • Login
  • English 
    • español
    • English

UniversidaddeCádiz

Área de Biblioteca, Archivo y Publicaciones
Communities and Collections
View Item 
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
  •   RODIN Home
  • Institutos de Investigación
  • Instituto de Investigación en Biomoléculas INBIO
  • Artículos Científicos INBIO
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cloning and molecular ontogeny of digestive enzymes in fed and food-deprived developing gilthead seabream (Sparus aurata) larvae

Thumbnail
Identificadores

URI: http://hdl.handle.net/10498/20382

DOI: 10.1016/j.cbpb.2015.09.006

Files
2016-Cloning and molecular ontogeny of digestive enzymes in fed and food-deprived developing gilthead seabream (Sparus aurata) larvae.pdf (3.574Mb)
Statistics
View statistics
Metrics and citations
 
Share
Export
Export reference to MendeleyRefworksEndNoteBibTexRIS
Metadata
Show full item record
Author/s
Mata-Sotres, José Antonio; Martos-Sitcha, Juan Antonio; Yúfera, Manuel; Martínez-Rodríguez, Gonzalo; Astola González, Antonio
Date
2015-09
Department
Bioquímica y Biología Molecular, Microbiología, Medicina Preventiva, Salud Pública
Source
Comparative Biochemistry and Physiology, Part B 191 (2016) 53–65
Abstract
We have determined the expression pattern of key pancreatic enzymes precursors (trypsinogen, try; chymotrypsinogen, ctrb; phospholipase A2, pla2; bile salt-activated lipase, cel; and α-amylase, amy2a) during the larval stage of gilthead seabream (Sparus aurata) up to 60 days after hatching (dph). Previously, complete sequences of try, cel, and amy2awere cloned and phylogenetically analyzed. One new isoformwas found for cel transcript (cel1b). Expression of all enzyme precursors was detected before the mouth opening. Expression of try and ctrb increased during the first days of development and then maintained high values with some fluctuations during the whole larval stage. The prolipases pla2 and cel1b increased from first-feeding with irregular fluctuation until the end of the experiment. Contrarily, cel1a maintained lowexpression values duringmost of the larval stage increasing at the end of the period. Nevertheless, cel1a expression was negligible as compared with cel1b. The expression of amy2a sharply increased during the first week followed by a gradual decrease. In addition, a fooddeprivation experiment was performed to find the differences in relation to presence/absence of gut content after the opening of the mouth. The food-deprived larvae died at 10 dph. The expression levels of all digestive enzymes increased up to 7 dph, declining sharply afterwards. This expression pattern up to 7 dphwas the same observed in fed larvae, confirming the genetic programming during the early development.Main digestive enzymes in gilthead seabream larvae exhibited the same expression profiles than other marine fish with carnivorous preferences in their juvenile stages.
Subjects
digestive enzymes; fish larvae; gene expression; gilthead seabream; sparus aurata
Collections
  • Artículos Científicos [3022]
  • Articulos Científicos Biomedicina [79]
  • Artículos Científicos INBIO [198]
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Browse

All of RODINCommunities and CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics

Información adicional

AboutDeposit in RODINPoliciesGuidelinesRightsLinksStatisticsNewsFrequently Asked Questions

RODIN is available through

OpenAIREOAIsterRecolectaHispanaEuropeanaBaseDARTOATDGoogle Academic

Related links

Sherpa/RomeoDulcineaROAROpenDOARCreative CommonsORCID

RODIN está gestionado por el Área de Biblioteca, Archivo y Publicaciones de la Universidad de Cádiz

Contact informationSuggestions