Faster through training: The anammox case

Abstract

Anaerobic ammonium oxidizing (anammox) bacteria based technologies are widely applied for nitrogen removal from warm (25-40 °C) wastewater with high ammonium concentrations (~1 gNH4-N L-1). Extension of the operational window of this energy and resource efficient process is restricted by the "supposed" low growth rate of the responsible microorganisms. Here we demonstrate that the maximum specific growth rate (μmax) of anammox bacteria can be increased to a μmax value of 0.33 d-1 by applying a novel selection strategy based on the maximization of the electron transfer capacity in a membrane bioreactor. This value is four times higher than the highest previously reported value. The microbial community was strongly dominated by anammox bacteria closely related (99%) to Candidatus Brocadia sp.40 throughout the experiment. The results described here demonstrate the remarkable capacity of a phylogenetically stable anammox community to adjust its growth rate in response to a change in the cultivation conditions imposed.