Pitfalls in the ABTS peroxidase activity test: Interference of photochemical processes

Identificadores

URI: http://hdl.handle.net/10498/39694

DOI: 10.1021/acs.inorgchem.8b02525

ISSN: 0020-1669

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Author/s
Liberato González, Andrea; Fernández-Trujillo Rey, María JesúsAuthority UCA; Máñez Muñoz, María ÁngelesAuthority UCA; Maneiro, Marcelino; Rodríguez-Silva, Laura; García Basallote, ManuelAuthority UCA
Date
2018
Department
Ciencia de los Materiales e Ingeniería Metalúrgica y Química Inorgánica
Source
Inorganic Chemistry 2018, vol. 57, n. 23, pp. 14471−14475
Abstract
ABTS oxidation to form its radical cation in the presence of H2O2 is frequently used as a test for determining the peroxidase activity of enzyme mimics. Detailed studies using salen-type Mn(III) complexes show that photochemical processes involving H2O2, ABTS and the complex itself, can lead to erroneous results. The capability of the complexes to act as ˙OH scavengers can be also relevant when the mechanism of their biological activity is considered
Subjects
ABTS; Photochemistry; Kinetics
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This work is under a Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 Internacional