Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation

Abstract

The impregnation of the polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with mango leaves extract (MLE) was carried out using the Supercritical Solvent Impregnation method. The impregnation loads, antioxidant compounds and physical properties of impregnated polymers were investigated with the variation of operating parameters (temperature (35 and 55 ◦ C), pressure (10, 20 and 30MPa) and depressurization rate (0.1 and 5MPa/min)). Furthermore, the influence of varying impregnation times (1, 3, 6, 10, 24h) with 30MPa, 35 ◦ Cand5MPa/minwasalsoinvestigated and the antimicrobial capacity (AC) was determined. The results showed a decrease of the impregnation loads, antioxidant and antimicrobial capacities with increasing impregnation time. At 30MPa, 35 ºC and 5MPa/min with an impregnation time of 1h, impregnation loads and antioxidant capacities of 2.82 ±0.04mg MLE/100mg PHB-HV and 2.77±0.10mg AC/100mg PHB-HV, respectively, and a growth inhibition percentage against Staphylococcus aureus and Escherichia coli bacteria greater than 60 % were obtained.